Six Respiratory Bacterial Pathogens Nucleic Acid Detection kit (multiplex fluorescence PCR method)

【Product Name】Six Respiratory Bacterial Pathogens Nucleic Acid Detection kit (multiplex fluorescence PCR method)

【Packaging Specification】 Forty eight servings per box

【Expected use】This kit is used for in vitro qualitative detection of nucleic acids of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii in human sputum samples. This product cannot distinguish the specific types of pathogens. 

The examination and measurement results can be used as auxiliary diagnosis for suspected pathogenic bacterial infections in the lower respiratory tract. The test results only provide clinical reference and cannot be used alone as a basis for accurate diagnosis or exclusion of cases.

【Testing Principle】

This kit is based on TaqMan probe method fluorescence PCR technology and fluorescence PCR probe melting curve technology. In a single fluorescence channel, TaqMan probe method fluorescence PCR technology is used to detect the target sequence of one pathogen, and another pathogen target sequence is detected using fluorescence PCR probe melting curve technology; 

Realize simultaneous detection of two targets in a single fluorescent channel. This test kit is designed with specific primers and probes for the conserved region of the nucleic acid of the pathogen to be detected, matched with

PCR reaction solution and other components are used to rapidly detect nucleic acids of respiratory pathogens in samples through changes in fluorescence signals. At the same time, specific primers and probes targeting conserved regions of human housekeeping genes were designed as internal references to monitor sample collection, transportation, DNA extraction, and amplification processes,

 as well as to control the quality of false negative results caused by in tube inhibition. This reagent kit contains dUTP UDG anti contamination measures in its reaction system, which can effectively prevent contamination of amplification products in the laboratory.